Keynote Speakers

Dr. Hai Chi, Associate Professor

Lab. of Seafood Safety Control and Processing, East China Sea Fisheries Research Institute, Chinese Academy Fishery Sciences, Shanghai, China

Biography: Dr. Hai Chi is an Associate Professor in Lab. of Seafood Safety Control and Processing at East China Sea Fisheries Research Institute, Chinese Academy Fishery Sciences, Shanghai, China. Dr. Hai Chi was financially supported by China Scholarship Council, and conducted his Ph.D. work at Lab. of Molecular Microbiology at Norwegian University of Life Sciences in 2012. On completion of his Ph.D., Dr. Hai Chi now mainly focuses on the food quality and safety control, foodborne pathogens monitoring and inhibition, antimicrobial peptides (bacteriocins) and their applications on the seafood and aquaculture. Till now, Dr. Hai Chi has published approximately 100 academic papers, 8patents in China, and obtained scientific rewards from Shanghai and National bureau of oceanography.

Topic: Identification and Characterization of a Novel Bacteriocin Produced by Enterococcus faecium Y3

Abstract: Enterocins are ribosomally synthesized antimicrobial peptides produced by Enterococcus faecalis and they show the characteristics of salt tolerance, high temperature tolerance and easy hydrolysis by protease. Enterocins generally show a better antimicrobial effect against close relative species and they have high specificity for their target bacteria. Therefore, the inhibitory effect of Enterocin on the remnants of specific pathogenic bacteria is of great significance for food safe and regulation of intestinal diseases. In this study, a bacteriocin produced by Ent. faecalis DH9003 that can inhibit common food-borne pathogens were screened from goat milk, and their molecular and biochemical characteristics were analyzed. The molecular of the purified Enterocin Y3 was 2.0kDa by analysis of MALDI-TOF-MS. The novel bacteriocin (termed as Enterocin Y3) possessed antimicrobial abilities to dissolve cell wall membrane, and lead to leakage of content of the cells by observing the scanning electron microscopy and transmission electron microscopy.